p38 alpha [p Thr180, p Tyr182] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-138

Novus Biologicals, part of Bio-Techne
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NB500-138
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human, Mouse, Rat, Fish - Danio rerio (Zebrafish)

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow (Intracellular), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry Control, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p38 alpha Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 conjugated to KLH. Accession # P70618

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 29692779). Mouse reactivity reported in scientific literature (PMID: 24105651).

Modification

p Thr180, p Tyr182

Localization

Cytoplasmic and Nuclear.

Specificity

Specific for endogenous levels of the ~39 kDa p38 alpha protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p38 alpha [p Thr180, p Tyr182] Antibody

Western Blot: p38 alpha [p Thr180, p Tyr182] Antibody [NB500-138]

Western Blot: p38 alpha [p Thr180, p Tyr182] Antibody [NB500-138]

Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Hela cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the -39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.
Immunohistochemistry-Paraffin: p38 alpha [p Thr180, p Tyr182] Antibody [NB500-138]

Immunohistochemistry-Paraffin: p38 alpha [p Thr180, p Tyr182] Antibody [NB500-138]

Immunohistochemistry-Paraffin: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red.
Western Blot: p38 alpha [p Thr180, p Tyr182] Antibody [NB500-138]

Western Blot: p38 alpha [p Thr180, p Tyr182] Antibody [NB500-138]

Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182.
View 1 more images

Applications for p38 alpha [p Thr180, p Tyr182] Antibody

Application
Recommended Usage

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Western Blot

1:1000
Application Notes
Use in Flow-intracellular reported in scientific literature (PMID: 24105651).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p38 alpha

P38 is a reactivating kinase that is a member of the MAP kinase family. It is activated in response to inflammatory cytokines, endotoxins, and osmotic stress. It is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002).

Alternate Names

MAPK14, SAPK2a

Entrez Gene IDs

1432 (Human)

Gene Symbol

MAPK14

UniProt

Q16539

Additional p38 alpha Products

Product Documents for p38 alpha [p Thr180, p Tyr182] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p38 alpha [p Thr180, p Tyr182] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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