p38 gamma/SAPK3 Antibody (OTI10E1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47852

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10E1

Concentration

1 mg/ml

View all p38 gamma Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human MAPK12 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens mitogen-activated protein kinase 12 (MAPK12).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p38 gamma/SAPK3 Antibody (OTI10E1)

Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]

Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]

Western Blot: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - hHEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAPK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAPK3.
Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]

Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]

Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - Staining of paraffin-embedded Human pancreas tissue using anti-SAPK3 mouse monoclonal antibody.
Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]

Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]

Flow Cytometry: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAPK3 antibody, and then analyzed by flow cytometry.
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Applications for p38 gamma/SAPK3 Antibody (OTI10E1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500

Reviewed Applications

Read 1 review rated 5 using NBP1-47852 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p38 gamma

Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes.

Alternate Names

MAPK12

Entrez Gene IDs

6300 (Human)

Gene Symbol

MAPK12

Additional p38 gamma Products

Product Documents for p38 gamma/SAPK3 Antibody (OTI10E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p38 gamma/SAPK3 Antibody (OTI10E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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