P4HA1 Antibody (4F1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43012
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4F1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
prolyl 4-hydroxylase, alpha polypeptide I - (Uniprot# P13674)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for P4HA1 Antibody (4F1)
Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody (4F1) [NBP3-43012] -
Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody (4F1) [NBP3-43012] - Immunofluorescent analysis of (-20℃ Ethanol ) fixed PC-3 cells using (P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)Western Blot: P4HA1 Antibody (4F1) [NBP3-43012] -
Western Blot: P4HA1 Antibody (4F1) [NBP3-43012] - PC-3 cells were subjected to SDS PAGE followed by western blot with (P4HA1 antibody) at dilution of 1:1000Immunohistochemistry: P4HA1 Antibody (4F1) [NBP3-43012] -
Immunohistochemistry: P4HA1 Antibody (4F1) [NBP3-43012] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (P4HA1 Antibody) at dilution of 1:500 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).Applications for P4HA1 Antibody (4F1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:500
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: P4HA1
Long Name
Prolyl 4-Hydroxylase Subunit Alpha 1
Alternate Names
4-PH Alpha-1, P4HA
Gene Symbol
P4HA1
Additional P4HA1 Products
Product Documents for P4HA1 Antibody (4F1)
Product Specific Notices for P4HA1 Antibody (4F1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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