P4HA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00005033-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Finch

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all P4HA1 Antibodies »

Product Specifications

Immunogen

P4HA1 (NP_001017962.1, 1 a.a. - 534 a.a.) full-length human protein. MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Reactivity Notes

Rat reactivity reported in customer review.

Specificity

P4HA1 - procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for P4HA1 Antibody

Western Blot: P4HA1 Antibody [H00005033-B01P]

Western Blot: P4HA1 Antibody [H00005033-B01P]

Western Blot: P4HA1 Antibody [H00005033-B01P] - Analysis of P4HA1 expression in transfected 293T cell line by P4HA1 polyclonal antibody. Lane 1: P4HA1 transfected lysate(58.74 KDa). Lane 2: Non-transfected lysate.
Western Blot: P4HA1 Antibody [H00005033-B01P]

Western Blot: P4HA1 Antibody [H00005033-B01P]

Western Blot: P4HA1 Antibody [H00005033-B01P] - P4HA1 MaxPab polyclonal antibody. Western Blot analysis of P4HA1 expression in A-431.

Applications for P4HA1 Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 5 using H00005033-B01P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: P4HA1

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Long Name

Prolyl 4-Hydroxylase Subunit Alpha 1

Alternate Names

4-PH Alpha-1, P4HA

Entrez Gene IDs

5033 (Human)

Gene Symbol

P4HA1

UniProt

NP_001017962.1 (Human)

Additional P4HA1 Products

Product Documents for P4HA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for P4HA1 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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