I would like to know how you set the lot number of these antibodies. When does the lot number change and what is the difference between lots. Is every lot being QC tested? Or dependent on the host animals (in polyclonal)?

Name: p53 Antibody (Pab1801)
Brand: Novus Biologicals
SKU: NB200-104
Rating: 5 (3 reviews)

The lot numbers for our products, including NB200-104 and NB200-171, change with every purification or production. So each lot number will refer to the particular purification batch (if the antibody is provided purified) or production batch (if the antibody is not purified). The antibodies are QC tested every lot to ensure specificity and reactivity.

p53 Antibody (Pab1801)

Novus Biologicals, part of Bio-Techne | Catalog # NB200-104

(3)

Citations (6)

Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Biotin

NB200-104B

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100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # Pab1801

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p53 Antibodies »

Product Specifications

Immunogen

Gel-purified fusion protein containing the coding region of human p53 expressed in E. coli.

Localization

Cytoplasm, Nucleus.

Specificity

Recognizes both wild-type and mutant forms of human p53 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p53 Antibody (Pab1801)

Western Blot: p53 Antibody (Pab1801) [NB200-104]

Western Blot: p53 Antibody (Pab1801) [NB200-104] - Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr 10% SDS PAGE.

Immunocytochemistry/ Immunofluorescence: p53 Antibody (Pab1801) [NB200-104]

Immunocytochemistry/Immunofluorescence: p53 Antibody (Pab1801) [NB200-104] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p53 stained by p53 antibody [Pab1801] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Immunohistochemistry-Paraffin: p53 Antibody (Pab1801) [NB200-104]

Immunohistochemistry-Paraffin: p53 Antibody (Pab1801) [NB200-104] - NCl-H1299 xenograft. p53 antibody [Pab1801] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Chromatin Immunoprecipitation: p53 Antibody (Pab1801) [NB200-104] - Immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell Lysate/extract treated with CPT 500nM for 6hr A. 5 ug preimmune mouse IgG B. 5 ug of p53 antibody (NB200-104) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Western Blot: p53 Antibody (Pab1801) [NB200-104]

Western Blot: p53 Antibody (PAb 1801) [NB200-104] - Lanes are as follows:. Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane 5 - 1.5ug of A549 lysate.

Immunohistochemistry-Paraffin: p53 Antibody (Pab1801) [NB200-104]

Immunohistochemistry-Paraffin: p53 Antibody (Pab1801) [NB200-104] - p53 Antibody (PAb 1801) [NB200-104] - Tumor tissues from human medulloblastoma cells xenografted in the mouse cerebellum stained with p53 antibody. Image submitted by a verified customer review.

Immunoprecipitation: p53 Antibody (Pab1801) [NB200-104]

Immunoprecipitation: p53 Antibody (Pab1801) [NB200-104] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (NB200-104). (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by NB200-104. EasyBlot anti-Mouse IgG Kit was used in Western blot.

Western Blot: p53 Antibody (Pab1801) [NB200-104] -

Western Blot: p53 Antibody (Pab1801) [NB200-104] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab1801] (NB200-104) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

View 5 more images

Applications for p53 Antibody (Pab1801)

Application

Recommended Usage

Immunohistochemistry

1:100-1:100

Immunohistochemistry-Frozen

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

10 ug/mg

Application Notes

p53 Antibody (Pab1801) antibody validated for IHC-P from a verified customer review.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using NB200-104 in the following applications:

Immunohistochemistry-Paraffin (1 Review)
Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human)

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 Antibody (Pab1801)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for p53 Antibody (Pab1801)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
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p53 Antibody (Pab1801)

Novus Biologicals, part of Bio-Techne | Catalog # NB200-104

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all p53 Antibodies »

Product Specifications

Immunogen

Localization

Specificity

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for p53 Antibody (Pab1801)

Western Blot: p53 Antibody (Pab1801) [NB200-104]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (Pab1801) [NB200-104]

Immunohistochemistry-Paraffin: p53 Antibody (Pab1801) [NB200-104]

Western Blot: p53 Antibody (Pab1801) [NB200-104]

Immunohistochemistry-Paraffin: p53 Antibody (Pab1801) [NB200-104]

Immunoprecipitation: p53 Antibody (Pab1801) [NB200-104]

Western Blot: p53 Antibody (Pab1801) [NB200-104] -

Applications for p53 Antibody (Pab1801)

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Immunoprecipitation

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: p53

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional p53 Products

Product Documents for p53 Antibody (Pab1801)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for p53 Antibody (Pab1801)