p57 Kip2 Antibody (KIP2/8169R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20451
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # KIP2/8169R
Concentration
0.2 mg/ml
Product Summary for p57 Kip2 Antibody (KIP2/8169R)
Immunogen
Recombinant full-length human p57 Kip2 protein
Localization
Nucleus.
Specificity
Recognizes a protein of 57kDa, identified as p57 Kip2 It shows no cross-reaction with p27Kip1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Raji, HT29 or SK-BR3 cells. Human colon or prostate carcinomas.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for p57 Kip2 Antibody (KIP2/8169R)
p57 Kip2 Antibody (KIP2/8169R)
IHC analysis of formalin-fixed, paraffin-embedded human placenta. Strong nuclear staining using p57 Kip2 antibody (KIP2/8169R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.
p57 Kip2 Antibody (KIP2/8169R)
IHC analysis of formalin-fixed, paraffin-embedded human placenta. Strong nuclear staining using p57 Kip2 antibody (KIP2/8169R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for p57 Kip2 Antibody (KIP2/8169R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: p57 Kip2
Alternate Names
Beckwith-Wiedemann syndrome, BWS, cyclin-dependent kinase inhibitor 1C, cyclin-dependent kinase inhibitor 1C (p57, Kip2), Cyclin-dependent kinase inhibitor p57, KIP2BWCR, p57, p57Kip2, WBS
Gene Symbol
CDKN1C
Additional p57 Kip2 Products
Product Documents for p57 Kip2 Antibody (KIP2/8169R)
Product Specific Notices for p57 Kip2 Antibody (KIP2/8169R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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