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p63/TP73L Antibody (TP40/3980R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07453

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-07453-100ug
NBP3-07453-20ug

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # TP40/3980R

Concentration

0.2 mg/ml

Product Specifications

Immunogen

A synthetic peptide from the N-terminal of human p63/TP73Lprotein (Exact sequence is proprietary) (Uniprot: Q9H3D4 )

Localization

Nuclear

Specificity

p63 consists of two major isoforms-TAp63 and delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent TA domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, DNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive delta-N domain, which antagonizes the activity of TAp63 and p53. P40/3980R recognizes exclusively delta-Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.

Marker

Squamous, Basal & Myoepithelial Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08775)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for p63/TP73L Antibody (TP40/3980R)

Immunocytochemistry/ Immunofluorescence: p63/TP73L Antibody (TP40/3980R) [NBP3-07453]

Immunocytochemistry/ Immunofluorescence: p63/TP73L Antibody (TP40/3980R) [NBP3-07453]

Immunocytochemistry/Immunofluorescence: p63/TP73L Antibody (TP40/3980R) [NBP3-07453] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling p63/TP73L Recombinant Rabbit Monoclonal Antibody (TP40/3980R) followed by goat anti-rabbit IgG-CF488 (Green). Membrane stained with phalloidin (red).
Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP40/3980R) [NBP3-07453]

Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP40/3980R) [NBP3-07453]

Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP40/3980R) [NBP3-07453] - Formalin-fixed, paraffin-embedded human skin stained with p63/TP73L Recombinant Rabbit Monoclonal Antibody (TP40/3980R).
Flow Cytometry: p63/TP73L Antibody (TP40/3980R) [NBP3-07453]

Flow Cytometry: p63/TP73L Antibody (TP40/3980R) [NBP3-07453]

Flow Cytometry: p63/TP73L Antibody (TP40/3980R) [NBP3-07453] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Recombinant Rabbit Monoclonal Antibody (TP40/3980R) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).

Applications for p63/TP73L Antibody (TP40/3980R)

Application
Recommended Usage

Flow Cytometry

2-4 ug/million cells

Immunocytochemistry/ Immunofluorescence

2-4 ug/ml

Immunohistochemistry-Paraffin

2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p63/TP73L

The p63 gene, a homologue of the tumor suppressor p53, is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 and to the related p73 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis.

Long Name

Tumor Protein 63

Alternate Names

EEC3, KET, OFC8, p40, p51, p73L, SHFM4, TP63, TP73L

Gene Symbol

TP63

UniProt

Additional p63/TP73L Products

Product Documents for p63/TP73L Antibody (TP40/3980R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p63/TP73L Antibody (TP40/3980R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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