p66 beta Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93311

Novus Biologicals, part of Bio-Techne
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NBP2-93311-0.02ml
NBP2-93311-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p66 beta Antibodies »

Product Summary for p66 beta Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GATAD2B (NP_065750.1). MDRMTEDALRLNLLKRSLDPADERDDVLAKRLKMEGHEAMERLKMLALLKRKDLANLEVPHELPTKQDGSGVKGYEEKLNGNLRPHGDNRTAGRPGKENINDEPVDMSARRSEPERGRLT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p66 beta Antibody - BSA Free

Western Blot: p66 beta AntibodyBSA Free [NBP2-93311]

Western Blot: p66 beta AntibodyBSA Free [NBP2-93311]

Western Blot: p66 beta Antibody [NBP2-93311] - Analysis of extracts of various cell lines, using p66 beta at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry-Paraffin: p66 beta Antibody - BSA Free [NBP2-93311]

Immunohistochemistry-Paraffin: p66 beta Antibody - BSA Free [NBP2-93311]

Immunohistochemistry-Paraffin: p66 beta Antibody [NBP2-93311] - Immunohistochemistry of paraffin-embedded mouse pancreas using p66 beta Rabbit pAb (NBP2-93311) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation: p66 beta Antibody - BSA Free [NBP2-93311]

Immunoprecipitation: p66 beta Antibody - BSA Free [NBP2-93311]

Immunoprecipitation: p66 beta Antibody [NBP2-93311] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug p66 beta antibody (NBP2-93311). Western blot was performed from the immunoprecipitate using p66 beta antibody (NBP2-93311) at a dilution of 1:1000.

Applications for p66 beta Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p66 beta

P66beta/GATAD2B interacts with MBD2 (methyl-CpG-binding domain 2) and MBD3 (methyl-CpG-binding domain 3) and participates in transcriptional repression in association with the MBD2/NuRD and MBD3/NuRD histone deacetylase complexes. P66beta/GATAD2B is SUMOylated in vivo and its repressive activity has been shown to be enhanced by SUMOylation. Alternate names for P66beta/GATAD2B include transcriptional repressor p66 beta, p66/p68, GATA zinc-finger domain-containing protein 2B, FLJ37346, and KIAA1150.

Alternate Names

FLJ37346, GATA zinc finger domain containing 2B, GATA zinc finger domain-containing protein 2B, KIAA1150, MGC138257, MGC138285, p66/p68, P66beta, RP11-216N14.6, transcription repressor p66 beta component of the MeCP1 complex, transcriptional repressor p66-beta

Gene Symbol

GATAD2B

Additional p66 beta Products

Product Documents for p66 beta Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for p66 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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