p67phox/NOXA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82543

Novus Biologicals, part of Bio-Techne
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NBP1-82543
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p67phox Antibodies »

Product Summary for p67phox/NOXA2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p67phox/NOXA2 Antibody

Western Blot: p67phox/NOXA2 Antibody [NBP1-82543]

Western Blot: p67phox/NOXA2 Antibody [NBP1-82543]

Western Blot: p67phox/NOXA2 Antibody [NBP1-82543] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
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Applications for p67phox/NOXA2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p67phox

NOXA2 encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in two transcript variants that encode the same protein.

Alternate Names

NCF2, NOXA2

Gene Symbol

NCF2

Additional p67phox Products

Product Documents for p67phox/NOXA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p67phox/NOXA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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