p67phox/NOXA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97097

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human p67phox/NOXA2 (Accession#: AAH01606.1; Met1-Val526)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for p67phox/NOXA2 Antibody

Western Blot: p67phox/NOXA2 Antibody [NBP2-97097]

Western Blot: p67phox/NOXA2 Antibody [NBP2-97097]

Western Blot: p67phox/NOXA2 Antibody [NBP2-97097] - Anti-p67phox/NOXA2 rabbit polyclonal antibody at 1:500 dilution. Lane A: THP1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa
Immunoprecipitation: p67phox/NOXA2 Antibody [NBP2-97097]

Immunoprecipitation: p67phox/NOXA2 Antibody [NBP2-97097]

Immunoprecipitation: p67phox/NOXA2 Antibody [NBP2-97097] - p67phox/NOXA2 was immunoprecipitated using: Lane A: 0.5 mg THP-1 Whole Cell Lysate. 2 ul anti-p67phox/NOXA2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-p67phox/NOXA2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa

Applications for p67phox/NOXA2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p67phox

NOXA2 encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in two transcript variants that encode the same protein.

Alternate Names

NCF2, NOXA2

Gene Symbol

NCF2

Additional p67phox Products

Product Documents for p67phox/NOXA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p67phox/NOXA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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