p70 S6 Kinase/S6K Antibody (5G9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22205

Novus Biologicals, part of Bio-Techne
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NBP2-22205
NBP2-22205-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G9

Format

BSA Free

Concentration

1 mg/ml

View all p70 S6 Kinase Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human p70 S6 Kinase/S6K expressed in E. Coli.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p70 S6 Kinase/S6K Antibody (5G9) - BSA Free

Western Blot: p70 S6 Kinase/S6K Antibody (5G9)BSA Free [NBP2-22205]

Western Blot: p70 S6 Kinase/S6K Antibody (5G9)BSA Free [NBP2-22205]

Western Blot: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Western blot analysis using S6K S6K mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)
Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) - BSA Free [NBP2-22205]

Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) - BSA Free [NBP2-22205]

Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Immunohistochemical analysis of paraffin-embedded muscle tissues using S6K mouse mAb with DAB staining.
Flow Cytometry: p70 S6 Kinase/S6K Antibody (5G9) - BSA Free [NBP2-22205]

Flow Cytometry: p70 S6 Kinase/S6K Antibody (5G9) - BSA Free [NBP2-22205]

Flow Cytometry: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Flow cytometric analysis of Jurkat cells using S6K mouse mAb (green) and negative control (red).
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Applications for p70 S6 Kinase/S6K Antibody (5G9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p70 S6 Kinase

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Gene Symbol

RPS6KB1

Additional p70 S6 Kinase Products

Product Documents for p70 S6 Kinase/S6K Antibody (5G9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p70 S6 Kinase/S6K Antibody (5G9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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