p70 S6 Kinase/S6K [p Ser411] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82120

Novus Biologicals, part of Bio-Techne
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NB100-82120
NB100-82120-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all p70 S6 Kinase Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase/S6K around the phosphorylation site of serine 411 (I-R-SP-P-R).

Modification

p Ser411

Specificity

Detects endogenous levels of p70 S6 Kinase/S6K only when phosphorylated at serine 411.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p70 S6 Kinase/S6K [p Ser411] Antibody

Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120]

Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120]

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Staining of methanol-fixed MCF7 cells using p70 S6 Kinase(Phospho-Ser411) antibody.

Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] -

Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using NB100-82120 (left) or the same antibody preincubated with blocking peptide(right).

Western Blot: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] -

Western Blot: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Analysis of extracts from MCF cells untreated or treated with EGF using NB100-82120

Applications for p70 S6 Kinase/S6K [p Ser411] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p70 S6 Kinase

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Entrez Gene IDs

6198 (Human); 72508 (Mouse); 83840 (Rat)

Gene Symbol

RPS6KB1

UniProt

P23443 (Human)

Additional p70 S6 Kinase Products

Product Documents for p70 S6 Kinase/S6K [p Ser411] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p70 S6 Kinase/S6K [p Ser411] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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