p70 S6 Kinase/S6K [p Thr449] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11951

Novus Biologicals, part of Bio-Techne
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NBP3-11951-100ul
NBP3-11951-25ul
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Key Product Details

Species Reactivity

Validated:

A. thaliana

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p70 S6 Kinase Antibodies »

Product Summary for p70 S6 Kinase/S6K [p Thr449] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 conjugated to KLH. (UniProt: P42818)

Modification

p Thr449

Specificity

Specific for endogenous levels of the ~53 kDa S6K1 protein phosphorylated at Thr449. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p70 S6 Kinase/S6K [p Thr449] Antibody

Western Blot: p70 S6 Kinase/S6K [p Thr449] Antibody [NBP3-11951]

Western Blot: p70 S6 Kinase/S6K [p Thr449] Antibody [NBP3-11951]

Western Blot: p70 S6 Kinase/S6K [p Thr 449] Antibody [NBP3-11951] - Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa p70 S6 Kinase/S6K phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).

Applications for p70 S6 Kinase/S6K [p Thr449] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: p70 S6 Kinase

S6K encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Gene Symbol

RPS6KB1

Additional p70 S6 Kinase Products

Product Documents for p70 S6 Kinase/S6K [p Thr449] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p70 S6 Kinase/S6K [p Thr449] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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