p73 Antibody (5B1288) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56674

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Co-Immunoprecipitation, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B1288

Format

BSA Free

Concentration

1.0 mg/ml

View all p73 Antibodies »

Product Specifications

Immunogen

This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule (NP_005418).

Specificity

It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73 (Costanzo, et al, 2002). Because of its reactivity pattern it may be regarded as anti-pan p73. NB100-56674 has been shown to recognize all the known alternative splicing variants of human and mouse p73 (Costanzo et al, 2002). The antibody does not cross react with p53.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p73 Antibody (5B1288) - BSA Free

Immunohistochemistry-Paraffin: p73 Antibody (5B1288) - BSA Free [NB100-56674]

Immunohistochemistry-Paraffin: p73 Antibody (5B1288) - BSA Free [NB100-56674]

Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - Human breast cancer section using 10ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.
Western Blot: p73 Antibody (5B1288)BSA Free [NB100-56674]

Western Blot: p73 Antibody (5B1288)BSA Free [NB100-56674]

Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of the expression level of p73 isoforms. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/article/11774) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: p73 Antibody (5B1288) - BSA Free [NB100-56674]

Immunohistochemistry-Paraffin: p73 Antibody (5B1288) - BSA Free [NB100-56674]

Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - Human breast cancer section using 10 ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.
View 2 more images

Applications for p73 Antibody (5B1288) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:20 - 1:1000. Use reported in scientific literature (Accardi et al)

Chromatin Immunoprecipitation (ChIP)

1:20-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000. Use reported in scientific literature (Sayan et al)

Immunohistochemistry

1:100 - 1:200. Use reported in scientific literature (PMID 19816568)

Immunohistochemistry-Paraffin

1:100 - 1:200

Immunoprecipitation

1:20 - 1:1000. Use reported in scientific literature (Sayan et al)

Western Blot

1-3 ug/ml
Application Notes
Immunoprecipitation details can be found in Sayan et al (2005). Endogenous expression of p73 has been detected in a variety of cell types; please refer to Product Citations for details regarding culture and treatment conditions. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p73

p73 was identified as a long-lost cousin of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein. Tumor BioMarker: Vella et al (2003) found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas p73 was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.

Long Name

Tumor Protein p73

Alternate Names

TP73

Entrez Gene IDs

7161 (Human); 22062 (Mouse)

Gene Symbol

TP73

UniProt

O15350 (Human)

Additional p73 Products

Product Documents for p73 Antibody (5B1288) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p73 Antibody (5B1288) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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