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p97/VCP Antibody (4G9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-39850

Novus Biologicals, part of Bio-Techne
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NBP1-39850

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G9

Format

BSA Free

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Recombinant human p97/VCP.

Specificity

Recombinant human p97/VCP protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for p97/VCP Antibody (4G9) - BSA Free

Western Blot: p97/VCP Antibody (4G9)BSA Free [NBP1-39850]

Western Blot: p97/VCP Antibody (4G9)BSA Free [NBP1-39850]

Western Blot: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]

Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI.
Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]

Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]

Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - Analysis of anti-VCP antibody with human prostate at concentration 5 ug/ml.

Applications for p97/VCP Antibody (4G9) - BSA Free

Application
Recommended Usage

Immunofluorescence

1-4 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
IHC: NBP1-39850 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2). Sourced from tissue culture supernatant.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VCP

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Entrez Gene IDs

7415 (Human)

Gene Symbol

VCP

UniProt

Additional VCP Products

Product Documents for p97/VCP Antibody (4G9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p97/VCP Antibody (4G9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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