p97/VCP Antibody (5H12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26257
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5H12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for p97/VCP Antibody (5H12)
Immunogen
A synthesized peptide derived from Human p97/VCP [UniProt P55072]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for p97/VCP Antibody (5H12)
Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] -
Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] - Immunoprecipitating p97/VCP in U251 whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26257 in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26257 (2ug) + U251 whole cell lysate (500ug).
Lane 3: U251 whole cell lysate (10ug).
Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] -
Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] -
Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for p97/VCP Antibody (5H12)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: VCP
Long Name
Valosin-containing Protein
Alternate Names
IBMPFD, TERA
Gene Symbol
VCP
Additional VCP Products
Product Documents for p97/VCP Antibody (5H12)
Product Specific Notices for p97/VCP Antibody (5H12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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