p97/VCP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1558
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Zebrafish
Cited:
Human, Mouse, Rat, Fish - Danio rerio (Zebrafish)
Predicted:
Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 806) of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).
Reactivity Notes
Zebrafish reactivity reported in scientific literature (PMID: 30010465). Mouse, Rat reactivity reported in scientific literature (PMID: 20833645).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p97/VCP Antibody
Western Blot: p97/VCP Antibody [NB100-1558]
Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY license.Western Blot: p97/VCP Antibody [NB100-1558]
Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Western Blot: p97/VCP Antibody [NB100-1558]
Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Applications for p97/VCP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunoprecipitation
2-5 ug/mg of lysate
Western Blot
1:2000 - 1:10000
Application Notes
ICC/IF reactivity reported in (PMID: 23297223), IHC-Frozen reactivity reported in (PMID: 20833645).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: VCP
Long Name
Valosin-containing Protein
Alternate Names
IBMPFD, TERA
Gene Symbol
VCP
UniProt
Additional VCP Products
Product Documents for p97/VCP Antibody
Product Specific Notices for p97/VCP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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