p97/VCP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81619

Novus Biologicals, part of Bio-Techne
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NBP1-81619
NBP1-81619-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VCP Antibodies »

Product Summary for p97/VCP Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p97/VCP Antibody

Western Blot: p97/VCP Antibody [NBP1-81619]

Western Blot: p97/VCP Antibody [NBP1-81619]

Western Blot: p97/VCP Antibody [NBP1-81619] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody [NBP1-81619]

Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody [NBP1-81619]

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody [NBP1-81619] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81619]

Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81619]

Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81619] - Staining of human skeletal muscle shows moderate nuclear and cytoplasmic positivity in a subset of myocytes.
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Applications for p97/VCP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VCP

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Entrez Gene IDs

7415 (Human); 269523 (Mouse); 116643 (Rat)

Gene Symbol

VCP

UniProt

P55072 (Human)

Additional VCP Products

Product Documents for p97/VCP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p97/VCP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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