PA1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76239

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PA1 Antibodies »

Product Summary for PA1 Antibody - BSA Free

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human GAS. The immunogen is located within the last 50 amino acids of GAS. Amino Acid Squence: LDSEDPSPASPPLRS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PA1 Antibody - BSA Free

Western Blot: PA1 AntibodyBSA Free [NBP1-76239]

Western Blot: PA1 AntibodyBSA Free [NBP1-76239]

Western Blot: PA1 Antibody [NBP1-76239] - EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide with PA1 antibody at 1ug/mL.

Applications for PA1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PA1

Steroid receptor co-activators (SRCs) were initially described as nuclear receptor transcription co-activators, but they have recently been determined to co-regulate transcription initiated by other transcription factors. GAS is a recently identified glutamate-rich protein that interacts with SRC1, but not GRIP1 or AIB1, the other two members of the SRC family. GAS can also interact with the alpha subunit of the estrogen receptor (ERalpha), but not other receptors such as the retinoic acid receptor a, suggesting the interaction between GAS and ERalpha is relatively specific. Depletion of GAS by RNA interference in MCF7 cells led to a decrease in the mRNA and protein levels of ER target genes such as pS2, c-Myc and cyclin D1, indicating the role of GAS in the regulation of ER target genes. GAS has also been found to associate with an SET1-like methyltransferase complex specific for H3K4 methylation, suggesting that GAS has multiple roles in transcriptional regulation.

Alternate Names

chromosome 16 open reading frame 53, FLJ22459, GAS, glutamate-rich coactivator interacting with SRC1/NCOA1, MGC4606, PA1, PAXIP1-associated protein 1, PTIP-associated 1 protein, PTIP-associated protein 1

Gene Symbol

PAGR1

UniProt

NP_078792 (Human)

Additional PA1 Products

Product Documents for PA1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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