PA28 Activator alpha Subunit/PSME1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83121

Novus Biologicals, part of Bio-Techne
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NBP1-83121
NBP1-83121-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PA28 Activator alpha Subunit Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PA28 Activator alpha Subunit/PSME1 Antibody

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121]

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121]

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121]

Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121]

Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody [NBP1-83121] - Staining of human tonsil shows strong cytoplasmic positivity in germinal and non-germinal center cells.

Applications for PA28 Activator alpha Subunit/PSME1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

reported by internal validation

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 25918340). For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western internal validation: Human Multiple Myeloma as sample; separated by size; antibody dilution of 1:50; observed molecular weight was 37 kDa; detected by Chemiluminescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PA28 Activator alpha Subunit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 1

Alternate Names

11S Regulator Complex Subunit alpha, IFI5111, PSME1, REG alpha

Gene Symbol

PSME1

Additional PA28 Activator alpha Subunit Products

Product Documents for PA28 Activator alpha Subunit/PSME1 Antibody

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Certificate of Analysis

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Product Specific Notices for PA28 Activator alpha Subunit/PSME1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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