PA28 Activator gamma Subunit/PSME3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33498

Novus Biologicals, part of Bio-Techne
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NBP1-33498
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (94%), Chimpanzee (99%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PA28 Activator gamma Subunit Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PA28 Activator gamma Subunit/PSME3. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (88%).

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PA28 Activator gamma Subunit/PSME3 Antibody

Knockout Validated: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498]

Knockout Validated: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498]

Knockout Validated: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Knockout/KnockdownWild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498]

Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498]

Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498]

Immunocytochemistry/ Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498]

Immunocytochemistry/Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
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Applications for PA28 Activator gamma Subunit/PSME3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PA28 Activator gamma Subunit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified.

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 3

Alternate Names

PSME3, REG gamma

Entrez Gene IDs

10197 (Human)

Gene Symbol

PSME3

UniProt

P61289 (Human)

Additional PA28 Activator gamma Subunit Products

Product Documents for PA28 Activator gamma Subunit/PSME3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PA28 Activator gamma Subunit/PSME3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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