PABP Antibody (10E10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-6125

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Bovine, Canine, Drosophila (Negative), Mouse (Negative), Primate, Xenopus

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 10E10

Format

BSA Free

Concentration

1.0 mg/ml

View all PABP Antibodies »

Product Specifications

Immunogen

Recombinant human PABP protein. [Uniprot# P11940]

Reactivity Notes

Does not cross-react with Drosophilia melanogaster or Mouse. Not yet tested in other species.

Localization

Cytoplasmic. Shuttles between the cytoplasm and the nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PABP Antibody (10E10) - BSA Free

Western Blot: PABP Antibody (10E10)BSA Free [NB120-6125]

Western Blot: PABP Antibody (10E10)BSA Free [NB120-6125]

Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) - BSA Free [NB120-6125]

Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) - BSA Free [NB120-6125]

Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.
Flow Cytometry: PABP Antibody (10E10) - BSA Free [NB120-6125]

Flow Cytometry: PABP Antibody (10E10) - BSA Free [NB120-6125]

Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Applications for PABP Antibody (10E10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NB120-6125 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PABP

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.

Alternate Names

PAB1polyadenylate-binding protein 1, PABP, PABP-1, PABP1poly(A) binding protein, cytoplasmic 2, PABPC2, PABPL1, poly(A) binding protein, cytoplasmic 1, Poly(A)-binding protein 1, poly(A)-binding protein, cytoplasmic 2

Entrez Gene IDs

26986 (Human)

Gene Symbol

PABPC1

UniProt

P11940 (Human)

Additional PABP Products

Product Documents for PABP Antibody (10E10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PABP Antibody (10E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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