PABP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57869

Novus Biologicals, part of Bio-Techne
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NBP2-57869
NBP2-57869-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PABP Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: QTQNRAAYYPPSQIAQLRPSPRWTAQG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PABP Antibody

Western Blot: PABP Antibody [NBP2-57869]

Western Blot: PABP Antibody [NBP2-57869]

Western Blot: PABP Antibody [NBP2-57869] - Analysis using Anti-PABPC1 antibody NBP2-57869 (A) shows similar pattern to independent antibody NBP2-54722 (B).
Immunocytochemistry/ Immunofluorescence: PABP Antibody [NBP2-57869]

Immunocytochemistry/ Immunofluorescence: PABP Antibody [NBP2-57869]

Immunocytochemistry/Immunofluorescence: PABP Antibody [NBP2-57869] - Staining of human cell line RT4 shows localization to cytosol.
Western Blot: PABP Antibody [NBP2-57869]

Western Blot: PABP Antibody [NBP2-57869]

Western Blot: PABP Antibody [NBP2-57869] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
View 1 more images

Applications for PABP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

1:250 - 1:500
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PABP

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.

Alternate Names

PAB1polyadenylate-binding protein 1, PABP, PABP-1, PABP1poly(A) binding protein, cytoplasmic 2, PABPC2, PABPL1, poly(A) binding protein, cytoplasmic 1, Poly(A)-binding protein 1, poly(A)-binding protein, cytoplasmic 2

Gene Symbol

PABPC1

Additional PABP Products

Product Documents for PABP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PABP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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