PABPC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74593

Novus Biologicals, part of Bio-Techne
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NB100-74593
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen (Negative), Immunohistochemistry-Paraffin, Immunoprecipitation, Single Cell Western, Western Blot

Cited:

Immunohistochemistry-Paraffin, Single Cell Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PABPC4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human Poly(A)-binding protein 4 using the numbering given in entry NP_003810.1 (GeneID 8761).

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PABPC4 Antibody

Western Blot: PABPC4 Antibody [NB100-74593]

Western Blot: PABPC4 Antibody [NB100-74593]

Western Blot: PABPC4 Antibody [NB100-74593] - Detection of PABP4 on HeLa whole cell lysate (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) using NB100-74593. PABP4 was also immunoprecipitated by rabbit anti-PABP4 antibody NB100-74594.
Immunohistochemistry-Paraffin: PABPC4 Antibody [NB100-74593]

Immunohistochemistry-Paraffin: PABPC4 Antibody [NB100-74593]

Immunohistochemistry-Paraffin: PABPC4 Antibody [NB100-74593] - Section of human prostate carcinoma (left) and colon carcinoma (right). Affinity purified rabbit anti- PABP4 used at 1:200 (1 ug/mL). Detection by DAB.

Applications for PABPC4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

2-5 ug/mL Lysate

Single Cell Western

10 ug/mL

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in Single Cell Western reported in scientific literature (PMID: 31601799).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PABPC4

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.

Alternate Names

APP1FLJ43938, APP-1PABP-4, Inducible poly(A)-binding protein, IPABP, iPABPpoly(A)-binding protein, cytoplasmic 4 (inducible form), PABP4Activated-platelet protein 1, poly(A) binding protein, cytoplasmic 4 (inducible form), Poly(A)-binding protein 4, polyadenylate-binding protein 4

Entrez Gene IDs

8761 (Human); 230721 (Mouse)

Gene Symbol

PABPC4

UniProt

NP_003810.1 (Human)

Additional PABPC4 Products

Product Documents for PABPC4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PABPC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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