PABPN1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31805
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Porcine (100%), Rat (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for PABPN1 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PABPN1. The exact sequence is proprietary.
Reactivity Notes
Zebrafish (83%), Xenopus laevis (88%).
Localization
Nucleus/Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PABPN1 Antibody
Western Blot: PABPN1 Antibody [NBP1-31805]
Western Blot: PABPN1 Antibody [NBP1-31805] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE; antibody diluted at 1:1000.Immunocytochemistry/ Immunofluorescence: PABPN1 Antibody [NBP1-31805]
Immunocytochemistry/Immunofluorescence: PABPN1 Antibody [NBP1-31805] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PABPN1 protein stained by PABPN1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.Immunohistochemistry-Paraffin: PABPN1 Antibody [NBP1-31805]
Immunohistochemistry-Paraffin: PABPN1 Antibody [NBP1-31805] - Human colon carcinoma, using PABPN1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for PABPN1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Application Notes
IHC-FR- Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PABPN1
Alternate Names
Nuclear poly(A)-binding protein 1, OPMD, PAB2poly(A) binding protein II, PABII, PABP-2, PABP2polyadenylate-binding protein 2, poly(A) binding protein 2, poly(A) binding protein, nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, poly(A)-binding protein, nuclear 1, Polyadenylate-binding nuclear protein 1
Gene Symbol
PABPN1
Additional PABPN1 Products
Product Documents for PABPN1 Antibody
Product Specific Notices for PABPN1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.