PACSIN3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47339

Novus Biologicals, part of Bio-Techne
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NBP2-47339
NBP2-47339-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PACSIN3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KPWLKRLKEVEASKKSYHAARKDEKTAQTRESHAKADSAVSQEQLRKLQERVERCAKEAEKTKAQYEQTLAELHR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PACSIN3 Antibody

Western Blot: PACSIN3 Antibody [NBP2-47339]

Western Blot: PACSIN3 Antibody [NBP2-47339]

Western Blot: PACSIN3 Antibody [NBP2-47339] - Analysis using Anti-PACSIN3 antibody NBP2-47339 (A) shows similar pattern to independent antibody NBP2-48707 (B).
Immunocytochemistry/ Immunofluorescence: PACSIN3 Antibody [NBP2-47339]

Immunocytochemistry/ Immunofluorescence: PACSIN3 Antibody [NBP2-47339]

Immunocytochemistry/Immunofluorescence: PACSIN3 Antibody [NBP2-47339] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: PACSIN3 Antibody [NBP2-47339]

Immunohistochemistry-Paraffin: PACSIN3 Antibody [NBP2-47339]

Immunohistochemistry-Paraffin: PACSIN3 Antibody [NBP2-47339] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal and glial cells.

Applications for PACSIN3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PACSIN3

PACSIN3 (protein kinase C and casein kinase substrate in neurons protein 3) is a member of the PACSIN family, a group of adaptor protein that regulate vesicle trafficking via cytoskeletal alterations. PACSIN3 is primarily expressed in skeletal muscle and heart and has been shown to be specifically involved in the trafficking of GLUT 1 in adipocytes. PACSIN3 is also known as SH3 domain-containing 6511, syndapin III, and SDPIII.

Alternate Names

Endophilin I, FLJ17861, protein kinase C and casein kinase substrate in neurons 3, protein kinase C and casein kinase substrate in neurons protein 3, SDPIII, SH3 domain-containing protein 6511, syndapin III

Gene Symbol

PACSIN3

Additional PACSIN3 Products

Product Documents for PACSIN3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PACSIN3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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