PADI4 Antibody (OTI4H5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73226

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4H5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PADI4 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PADI4(NP_036519) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

73.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PADI4 Antibody (OTI4H5) - Azide and BSA Free

Western Blot: PADI4 Antibody (OTI4H5)Azide and BSA Free [NBP2-73226]

Western Blot: PADI4 Antibody (OTI4H5)Azide and BSA Free [NBP2-73226]

Western Blot: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.
Immunocytochemistry/ Immunofluorescence: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226]

Immunocytochemistry/ Immunofluorescence: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226]

Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.
Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226]

Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226]

Immunohistochemistry: PADI4 Antibody (OTI4H5) - Azide and BSA Free [NBP2-73226] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.
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Applications for PADI4 Antibody (OTI4H5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PADI4

In vivo expression of PADI4 correlated with the appearance of citrulline in histones. We have shown that PADI4 converts arginines in the histone H3 tail to citrulline, that PADI4 blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to eostrogen regulated transcription.

Long Name

Peptidyl Arginine Deiminase type IV

Alternate Names

HL-60 PAD, PAD, PAD4, PDI4

Gene Symbol

PADI4

Additional PADI4 Products

Product Documents for PADI4 Antibody (OTI4H5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PADI4 Antibody (OTI4H5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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