PADI4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87353

Novus Biologicals, part of Bio-Techne
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NBP1-87353
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PADI4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CEDDEVLDSEDLQDMSLMTLSTKTPKDFFTNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMVPGGKHNMDFYVEALAFPDTDFPGLITLTISLLD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PADI4 Antibody

Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353]

Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353]

Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Analysis in human bone marrow and kidney tissues. Corresponding PADI4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353]

Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353]

Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP1-87353 (A) shows similar protein distribution across tissues to independent antibody NBP2-38499 (B).
Western Blot: PADI4 Antibody [NBP1-87353]

Western Blot: PADI4 Antibody [NBP1-87353]

Western Blot: PADI4 Antibody [NBP1-87353] - Analysis in control (vector only transfected HEK293T lysate) and pADI4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Applications for PADI4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PADI4

In vivo expression of PADI4 correlated with the appearance of citrulline in histones. We have shown that PADI4 converts arginines in the histone H3 tail to citrulline, that PADI4 blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to eostrogen regulated transcription.

Long Name

Peptidyl Arginine Deiminase type IV

Alternate Names

HL-60 PAD, PAD, PAD4, PDI4

Gene Symbol

PADI4

Additional PADI4 Products

Product Documents for PADI4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PADI4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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