PADI4 Antibody

Western Blot: PADI4 Antibody [NBP3-18652]

Western Blot: PADI4 Antibody [NBP3-18652] - Figure 2. Western blot analysis using the antibody directed against PADI4 Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with the antibody against PADI4 , diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

ELISA: PADI4 Antibody [NBP3-18652]

ELISA: PADI4 Antibody [NBP3-18652] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against PADI4 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.