PAF1 Antibody (3U5C4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16185

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16185-100ul
NBP3-16185-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3U5C4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAF1 Antibodies »

Product Summary for PAF1 Antibody (3U5C4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAF1 (NP_061961.2). MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAF1 Antibody (3U5C4)

Western Blot: PAF1 Antibody (3U5C4) [NBP3-16185]

Western Blot: PAF1 Antibody (3U5C4) [NBP3-16185]

Western Blot: PAF1 Antibody (3U5C4) [NBP3-16185] - Western blot analysis of extracts of various cell lines, using PAF1 Rabbit mAb (NBP3-16185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: PAF1 Antibody (3U5C4) [NBP3-16185]

Immunohistochemistry-Paraffin: PAF1 Antibody (3U5C4) [NBP3-16185]

Immunohistochemistry-Paraffin: PAF1 Antibody (3U5C4) [NBP3-16185] - Immunohistochemistry of paraffin-embedded human appendix using PAF1 Rabbit mAb (NBP3-16185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for PAF1 Antibody (3U5C4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAF1

PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and CTR9 (MIM 609366) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]). The PAF complex also has a role in histone monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied by OMIM]

Alternate Names

F23149_1, FLJ11123, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae), Pancreatic differentiation protein 2, PD2hPAF1, RNA polymerase II-associated factor 1 homolog

Gene Symbol

PAF1

Additional PAF1 Products

Product Documents for PAF1 Antibody (3U5C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PAF1 Antibody (3U5C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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