Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PAF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAF1 Antibody

Western Blot: PAF1 Antibody [NB600-273]

Western Blot: PAF1 Antibody [NB600-273]

Western Blot: PAF1 Antibody [NB600-273] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPaf1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: PAF1 Antibody [NB600-273]

Immunoprecipitation: PAF1 Antibody [NB600-273]

Immunoprecipitation: PAF1 Antibody [NB600-273] - Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-273 used for IP at 3 ug per reaction. Paf1 was also immunoprecipitated by rabbit anti-Paf1 antibody NB600-274. For blotting immunoprecipitated Paf1, NB600-273 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PAF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000- 1:15000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PAF1

Paf1 has been shown to form a complex with Rtf1, Ctr9, Cdc73, and Leo1 in yeast that may mediate the interaction between the elongation factor Spt16/Pob3 (FACT in humans) and RNA polymerase II. This mediation suggests that this complex may have an important role in transcription elongation, thereby suggesting that Paf1, Rtf1, Ctr9, Cdc73 and Leo1 may be elongation factors.

Alternate Names

F23149_1, FLJ11123, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae), Pancreatic differentiation protein 2, PD2hPAF1, RNA polymerase II-associated factor 1 homolog

Entrez Gene IDs

54623 (Human)

Gene Symbol

PAF1

UniProt

Q9NUU9 (Human)

Additional PAF1 Products

Product Documents for PAF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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