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PAF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19675

Novus Biologicals, part of Bio-Techne
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NBP2-19675

Key Product Details

Species Reactivity

Validated:

Mouse, Zebrafish

Predicted:

Bovine (100%), Human (100%), Primate (100%), Rat (100%), Xenopus (93%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PAF1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF1. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (93%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAF1 Antibody

Western Blot: PAF1 Antibody [NBP2-19675]

Western Blot: PAF1 Antibody [NBP2-19675]

Western Blot: PAF1 Antibody [NBP2-19675] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.
PAF1 Antibody

Immunohistochemistry: PAF1 Antibody [NBP2-19675] -

Immunohistochemistry: PAF1 Antibody [NBP2-19675] - PAF1 antibody detects Paf1 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
PAF1 antibody (NBP2-19675) dilution: 1:200.

Applications for PAF1 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAF1

PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and CTR9 (MIM 609366) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]). The PAF complex also has a role in histone monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied by OMIM]

Alternate Names

F23149_1, FLJ11123, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae), Pancreatic differentiation protein 2, PD2hPAF1, RNA polymerase II-associated factor 1 homolog

Gene Symbol

PAF1

UniProt

Additional PAF1 Products

Product Documents for PAF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.