PAF49 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90084

Novus Biologicals, part of Bio-Techne
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NBP1-90084
NBP1-90084-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PAF49 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GNPPVTGPRSALAPNLLTSGKKKKEMQVTEAPVTQEAVNGHGALEVDMALGSPEMDVRKKKKKKNQQLKEPEAAGPVGTEPTVETLEPLGVLFPSTTKKRKKPKGK

Marker

Nucleolar Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAF49 Antibody

Western Blot: PAF49 Antibody [NBP1-90084]

Western Blot: PAF49 Antibody [NBP1-90084]

Western Blot: PAF49 Antibody [NBP1-90084] - Analysis in control (vector only transfected HEK293T lysate) and CD3EAP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PAF49 Antibody [NBP1-90084]

Immunocytochemistry/ Immunofluorescence: PAF49 Antibody [NBP1-90084]

Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP1-90084] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli fibrillar center & mitochondria.
Immunohistochemistry-Paraffin: PAF49 Antibody [NBP1-90084]

Immunohistochemistry-Paraffin: PAF49 Antibody [NBP1-90084]

Immunohistochemistry-Paraffin: PAF49 Antibody [NBP1-90084] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Applications for PAF49 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAF49

PAF49/CAST is subunit of RNA polymerase I. PAF49/CAST has been shown to interact with activator upstream binding factor (UBF) and plays an important role in rDNA transcription.

Alternate Names

A34.5, antisense to ERCC 1, Antisense to ERCC-1 protein, ASE-1ASE1, CASTMGC118851, CD3 epsilon associated protein, CD3e antigen, epsilon polypeptide associated protein, CD3e molecule, epsilon associated protein, CD3E-associated protein, CD3-epsilon-associated protein, DNA-directed RNA polymerase I subunit RPA34, PAF49RNA polymerase I-associated factor PAF49

Gene Symbol

CD3EAP

Additional PAF49 Products

Product Documents for PAF49 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PAF49 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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