PAG1 Antibody (0K5F4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15731

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15731-100ul
NBP3-15731-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0K5F4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAG1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 333-432 of human PAG1 (Q9NWQ8). SLTVPESTYTSIQGDPQRSPSSCNDLYATVKDFEKTPNSTLPPAGRPSEEPEPDYEAIQTLNREEEKATLGTNGHHGLVPKENDYESISDLQQGRDITRL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAG1 Antibody (0K5F4)

Western Blot: PAG1 Antibody (0K5F4) [NBP3-15731]

Western Blot: PAG1 Antibody (0K5F4) [NBP3-15731]

Western Blot: PAG1 Antibody (0K5F4) [NBP3-15731] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 1 (PAG1) antibody (NBP3-15731) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for PAG1 Antibody (0K5F4)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAG1

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Long Name

Phosphoprotein Associated with Glycosphingolipid Microdomains 1

Alternate Names

CBP

Gene Symbol

PAG1

Additional PAG1 Products

Product Documents for PAG1 Antibody (0K5F4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PAG1 Antibody (0K5F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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