PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15222
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3H12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Phospho-PAK1/2/3 (S144+S141+S139). UniProt ID (O75914/Q13153/Q13177).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12)
Western Blot: PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12) [NBP3-15222] - Positive WB detected in A549 whole cell lysate (treated with EGF or not). All lanes: PAK1/2/3 antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 65 KDa. Observed band size: 65 KDa.
Immunohistochemistry: PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12) [NBP3-15222] - IHC image of PAK1/2/3 antibody diluted at 1:100 and staining in paraffin-embedded rat brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Applications for PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: PAK1/2/3
Alternate Names
alpha-PAK, EC 2.7.11, EC 2.7.11.1, MGC130000, MGC130001, p21 protein (Cdc42/Rac)-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), p21-activated kinase 1, p65-PAK, PAK-1, PAKalpha, serine/threonine-protein kinase PAK 1, STE20 homolog, yeast
Gene Symbol
PAK1
Additional PAK1/2/3 Products
Product Documents for PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12)
Product Specific Notices for PAK1/2/3 [p Ser144, p Ser141, p Ser139] Antibody (3H12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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