PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-82131
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from Human PAK1/2/3 around the phosphorylation site of threonine 423/402/421 (R-S-TP-M-V).
Modification
p Thr423, p Thr402, p Thr421
Specificity
Detects endogenous levels of PAK1/2/3 only when phosphorylated at threonine 423/402/421.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody
![Immunocytochemistry/ Immunofluorescence: PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody [NB100-82131]](https://resources.bio-techne.com/images/products/PAK1-2-3-[p-Thr423--p-Thr402--p-Thr421]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82131-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody [NB100-82131]")
Immunocytochemistry/ Immunofluorescence: PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody [NB100-82131]
Immunocytochemistry/Immunofluorescence: PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody [NB100-82131] - Staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) antibody
Immunohistochemistry-Paraffin: PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody [NB100-82131] -
Immunohistochemistry-Paraffin: PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody [NB100-82131] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82131 (left) or the same antibody preincubated with blocking peptide(right).Applications for PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody
Application
Recommended Usage
Immunohistochemistry
1:50-1:100
Application Notes
Use in Western blot reported in scientific literature (PMID 26821597). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29066727).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PAK1/2/3
Alternate Names
alpha-PAK, EC 2.7.11, EC 2.7.11.1, MGC130000, MGC130001, p21 protein (Cdc42/Rac)-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), p21-activated kinase 1, p65-PAK, PAK-1, PAKalpha, serine/threonine-protein kinase PAK 1, STE20 homolog, yeast
Gene Symbol
PAK1
UniProt
Additional PAK1/2/3 Products
Product Documents for PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody
Product Specific Notices for PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...