PAK1 Antibody (4F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15141

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15141
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4F10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAK1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PAK1. UniProt ID (Q13153).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK1 Antibody (4F10)

Western Blot: PAK1 Antibody (4F10) [NBP3-15141]

Western Blot: PAK1 Antibody (4F10) [NBP3-15141]

Western Blot: PAK1 Antibody (4F10) [NBP3-15141] - Positive WB detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, ntera-2 whole cell lysate, Mouse brain tissue. All lanes: PAK1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 61, 62 KDa. Observed band size: 61 KDa.
Immunocytochemistry/ Immunofluorescence: PAK1 Antibody (4F10) [NBP3-15141]

Immunocytochemistry/ Immunofluorescence: PAK1 Antibody (4F10) [NBP3-15141]

Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4F10) [NBP3-15141] - Immunofluorescence staining of Hela Cells with at PAK1 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for PAK1 Antibody (4F10)

Application
Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: PAK1

p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Gene Symbol

PAK1

Additional PAK1 Products

Product Documents for PAK1 Antibody (4F10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PAK1 Antibody (4F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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