PAK1 [p Thr84] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29511

Novus Biologicals, part of Bio-Techne
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NBP2-29511
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Guinea Pig (100%), Primate (100%), Sheep (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAK1 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 conjugated to KLH. Accession # Q13153

Modification

p Thr84

Specificity

Specific for endogenous levels of the ~68 kDa PAK1 phosphorylated at Thr84. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAK1 [p Thr84] Antibody

Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511]

Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511]

Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for PAK1 [p Thr84] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAK1

PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at thr84 and bind to gelsolin (Chen et al, 2004).

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Entrez Gene IDs

5058 (Human)

Gene Symbol

PAK1

UniProt

Q13153

Additional PAK1 Products

Product Documents for PAK1 [p Thr84] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PAK1 [p Thr84] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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