Pallidin Antibody (15G2)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-13261

Novus Biologicals, part of Bio-Techne
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NB120-13261
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Hamster

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 15G2

Concentration

1.0 mg/ml

View all Pallidin Antibodies »

Product Summary for Pallidin Antibody (15G2)

Immunogen

Recombinant full length protein (Rat).

Reactivity Notes

Cross-reacts with Mouse, Rat and Dog. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Tubular early and recycling endosomes and endosomal vacuoles.

Specificity

Syntaxin 13 (15G2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Pallidin Antibody (15G2)

Western Blot: Pallidin Antibody (15G2) [NB120-13261]

Western Blot: Pallidin Antibody (15G2) [NB120-13261]

Western Blot: Pallidin Antibody (15G2) [NB120-13261] - Analysis of Syntaxin 13 (15G2), mAb : Lane 1: MW Marker, Lane 2: Rat Brain Lysate Lane 3: Mouse Brain Lysate, Lane 4: CHO-K1 Cell Lysate (Heat Shocked)
Immunohistochemistry-Paraffin: Pallidin Antibody (15G2) [NB120-13261]

Immunohistochemistry-Paraffin: Pallidin Antibody (15G2) [NB120-13261]

Immunohistochemistry-Paraffin: Pallidin Antibody (15G2) [NB120-13261] - Analysis of human placenta tissue stained with Syntaxin 13, mAb (15G2) at 20 ug/ml.

Applications for Pallidin Antibody (15G2)

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
WB: Detects a band of approximately 38 kDa. Additional bands of 27 and 37 kDa may also be recognized, and are believed to correspond to degradation products of syntaxin 13.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pallidin

Syntaxin 13 is an integral membrane protein that belongs to the t-SNARE family, a group of proteins involved in protein transport. Confocal immunofluoresence and electron microscopy studies have shown that syntaxin 13 is primarily localized to tubular early and recycling endosomes, where it colocalizes with transferrin receptor, and it is also localized in endosomal vacuoles. Syntaxin 13 has been found to be expressed in all tissues, with higher levels of the protein found in brain, lung, spleen, thymus and testes. Immunoprecipitation studies show that syntaxin 13 complexes with beta-SNAP, VAMP2/3, and SNAP25. The binding of this complex to alpha-SNAP and NSF is terminated in the presence of ATP. These results suggest that syntaxin 13 is a SNARE protein which mediates the recycling protein flow through tubulo vesicular recycling endosomes.

Alternate Names

PALLID, pallid (mouse) homolog, pallidin, Pallid protein homolog, pallidin, pallidin homolog (mouse), PAsyntaxin 13 binding protein 1, Syntaxin 13-interacting protein, syntaxin 13-interacting protein pallid

Entrez Gene IDs

100226 (Mouse)

Gene Symbol

BLOC1S6

Additional Pallidin Products

Product Documents for Pallidin Antibody (15G2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pallidin Antibody (15G2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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