PALS1/MPP5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-952

Novus Biologicals, part of Bio-Techne
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NB300-952
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PALS1/MPP5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence NKASPPFPLISNAQ-C, from the N Terminus of the protein sequence according to NP_071919.2; NP_001243479.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PALS1/MPP5 Antibody

Immunocytochemistry/ Immunofluorescence: PALS1/MPP5 Antibody [NB300-952]

Immunocytochemistry/ Immunofluorescence: PALS1/MPP5 Antibody [NB300-952]

Immunocytochemistry/Immunofluorescence: PALS1/MPP5 Antibody [NB300-952] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: PALS1/MPP5 Antibody [NB300-952]

Immunohistochemistry-Paraffin: PALS1/MPP5 Antibody [NB300-952]

Immunohistochemistry-Paraffin: PALS1/MPP5 Antibody [NB300-952] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: PALS1/MPP5 Antibody [NB300-952]

Flow Cytometry: PALS1/MPP5 Antibody [NB300-952]

Flow Cytometry: PALS1/MPP5 Antibody [NB300-952] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for PALS1/MPP5 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary testing showed a band at approx 75kDa in Human Cerebellum lysate after 1ug/ml antibody staining (calculated MW of 73.4kDa according to NP_001243479.1). Primary incubation 1 hour at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PALS1/MPP5

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001). MPP5 is a member of the p55-like MAGUK subfamily.

Long Name

Protein Associated with Lin Seven 1

Alternate Names

MPP5, Stardust

Entrez Gene IDs

64398 (Human)

Gene Symbol

MPP5

UniProt

NP_071919. (Human)

Additional PALS1/MPP5 Products

Product Documents for PALS1/MPP5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PALS1/MPP5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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