pan Cadherin Antibody (6N5P8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16509

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16509-100ul
NBP3-16509-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6N5P8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cadherin Antibodies »

Product Summary for pan Cadherin Antibody (6N5P8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 806-906 of human Cadherin-2 (CDH2) (P19022). IRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKKLADMYGGGDD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for pan Cadherin Antibody (6N5P8)

Western Blot: pan Cadherin Antibody (6N5P8) [NBP3-16509]

Western Blot: pan Cadherin Antibody (6N5P8) [NBP3-16509]

Western Blot: pan Cadherin Antibody (6N5P8) [NBP3-16509] - Western blot analysis of extracts of various cell lines, using pan Cadherin Rabbit mAb (NBP3-16509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: pan Cadherin Antibody (6N5P8) [NBP3-16509]

Immunohistochemistry-Paraffin: pan Cadherin Antibody (6N5P8) [NBP3-16509]

Immunohistochemistry-Paraffin: pan Cadherin Antibody (6N5P8) [NBP3-16509] - Immunohistochemistry of paraffin-embedded human thyroid cancer using pan Cadherin Rabbit mAb (NBP3-16509) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for pan Cadherin Antibody (6N5P8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cadherin

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Long Name

Cadherin Pan Specific

Alternate Names

Arc-1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDHE, cell-CAM 120/80, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVOE-Cadherin, Uvomorulin

Gene Symbol

CDH1

Additional Cadherin Products

Product Documents for pan Cadherin Antibody (6N5P8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for pan Cadherin Antibody (6N5P8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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