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Pan-Endothelium Marker [p Ser123, p Ser124] Antibody (HIS52) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00076

Novus Biologicals, part of Bio-Techne
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NBP2-00076
NBP2-00076-0.025mg

Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HIS52

Format

BSA Free

Concentration

0.5 mg/ml

Product Specifications

Immunogen

The immunogen for this antibody is Pan endothelium.

Reactivity Notes

Rat

Modification

p Ser123, p Ser124, p ser123, p ser124

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1-10 ug/ml
Application Notes
The HIS52 antibody has been tested by immunohistochemistry of rat frozen sections. The HIS52 antibody has been reported for use in flow cytometric analysis. No reactivity of the antibody was seen when applied in immunoblotting of PAGE-run lysates from endothelial cell cultures and stromal cell preparations. is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pan-Endothelium Marker

The HIS52 monoclonal antibody reacts with a molecule expressed on the surface of all rat endothelial cells, also known as Rat Endothelium Cell Antigen-1 (RECA-1). The exact nature of HIS52 antigen is, however, not known. HIS52 does not cross react with various species tested other than rat. This antibody is a very useful tool as a specific phenotypic marker for rat endothelium.

Additional Pan-Endothelium Marker Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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