pan-Synuclein Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13218

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Product Datasheet / COA

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (91%), Chimpanzee (93%), Human (94%), Porcine (92%), Sheep (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Summary for pan-Synuclein Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of Rat pan-Synuclein. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%), Chicken (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for pan-Synuclein Antibody

Western Blot: pan-Synuclein Antibody [NBP3-13218]

Western Blot: pan-Synuclein Antibody [NBP3-13218] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with pan-Synuclein antibody (NBP3-13218) diluted at 1:1000.

Immunocytochemistry/ Immunofluorescence: pan-Synuclein Antibody [NBP3-13218]

Immunocytochemistry/Immunofluorescence: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: pan-Synuclein protein stained by pan-Synuclein antibody (NBP3-13218) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: pan-Synuclein Antibody [NBP3-13218]

Immunohistochemistry-Frozen: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: pan-Synuclein protein stained by pan-Synuclein antibody (NBP3-13218) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.

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Applications for pan-Synuclein Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: pan-Synuclein

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function (1).

Alternate Names

Lewy body) 4, MGC110988, non A-beta component of AD amyloid, Non-A beta component of AD amyloid, non-A4 component of amyloid, Non-A4 component of amyloid precursor, PARK4, synuclein, alpha (non A4 component of amyloid precursor)

Gene Symbol

SNCA

Additional pan-Synuclein Products

Product Documents for pan-Synuclein Antibody

Product Datasheets

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COA

Product Specific Notices for pan-Synuclein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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