Pancreatic Islet Lysate Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44126
Key Product Details
Species Reactivity
Porcine
Applications
Sandwich ELISA
Label
Unconjugated
Antibody Source
Polyclonal Canine IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells (Uniprot: P01300)
Reactivity Notes
Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates
Clonality
Polyclonal
Host
Canine
Isotype
IgG
Description
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for Pancreatic Islet Lysate Antibody
Application
Recommended Usage
Sandwich ELISA
1:50 -1:200
Application Notes
This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Pancreatic Islet Lysate
Alternate Names
HGNC:9327;IOPPP;panceatic polypeptide;Pancreatic prohormone percursor;PPA1;SID6-8061
UniProt
Additional Pancreatic Islet Lysate Products
Product Documents for Pancreatic Islet Lysate Antibody
Product Specific Notices for Pancreatic Islet Lysate Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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