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Pancreatic trypsin inhibitor Antibody (35) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23590

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 35

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Aprotinin from bovine lung

Epitope

The epitope of Clone 35 is different from the epitopes of Clone 18 and Clone 21

Specificity

Clone 35 binds bovine aprotinin immobilized on a solid phase or free in solution.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Pancreatic trypsin inhibitor Antibody (35) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-23590 (as capture antibody coated at 1 ug/mL) forms an antibody pair with NBP2-23588 (as biotinylated detection antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 ug/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-based buffers are unsuitable for this assay and 0.2 M glycine sodium buffer, pH 8.8, is recommended for washing and dilution buffers.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Pancreatic trypsin inhibitor

Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury

Alternate Names

Aprotinin, Basic protease inhibitor, BPI, BPTI, PTI, PTI pancreatic trypsin inhibitor

Gene Symbol

PTI

Additional Pancreatic trypsin inhibitor Products

Product Documents for Pancreatic trypsin inhibitor Antibody (35) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pancreatic trypsin inhibitor Antibody (35) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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