Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PAPD1 Antibodies »

Product Summary for PAPD1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 532 and 582 of human PAP-Associated Domain-Containing Protein 1 using the numbering given in entry NP_060579.3 (GeneID 55149).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAPD1 Antibody

Western Blot: PAPD1 Antibody [NBP2-22258]

Western Blot: PAPD1 Antibody [NBP2-22258]

Western Blot: PAPD1 Antibody [NBP2-22258] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PAPD1 Antibody [NBP2-22258]

Immunoprecipitation: PAPD1 Antibody [NBP2-22258]

Immunoprecipitation: PAPD1 Antibody [NBP2-22258] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22258 used for IP at 6 ug/mg lysate. PAPD1 was also immunoprecipitated by another rabbit anti-PAPD1 antibody. For blotting immunoprecipitated PAPD1, NBP2-22258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PAPD1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PAPD1

Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA

Alternate Names

EC 2.7.7.19, FLJ10486, mitochondrial poly(A) polymerase, mtPAP, PAP, PAP associated domain containing 1, PAP-associated domain-containing protein 1, PAPD1SPAX4, poly(A) RNA polymerase, mitochondrial, Polynucleotide adenylyltransferase, Terminal uridylyltransferase 1, TUTase 1

Gene Symbol

MTPAP

UniProt

NP_060579.3 (Human)

Additional PAPD1 Products

Product Documents for PAPD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAPD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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