PAPOLA Antibody

Western Blot: PAPOLA Antibody [NBP3-18653]

Western Blot: PAPOLA Antibody [NBP3-18653] - Figure 2. Western blot analysis using the antibody directed against PAPOLA Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against PAPOLA diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.

ELISA: PAPOLA Antibody [NBP3-18653]

ELISA: PAPOLA Antibody [NBP3-18653] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PAPOLA . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.