PAPOLG Antibody

Immunoprecipitation: PAPOLG Antibody [NBP1-30060]

Immunoprecipitation: PAPOLG Antibody [NBP1-30060] - WCL from HeLa cells. NBP1-30060 used for IP at 10 mcg/mg lysate. PAP-gamma was also IPed by rabbit anti-PAP-gamma antibody NBP1-30061. For blotting IPed PAP-gamma, NBP1-30061 was used at 1 mcg/ml.

Knockdown Validated: PAPOLG Antibody [NBP1-30060]

Knockdown Validated: PAPOLG Antibody [NBP1-30060] - An internal A-tract does not promote splicing in the absence of a 3'-terminal poly(A) tail. Western blot analysis of PAPalpha and PAPgamma (PAPOLG) proteins in cells treated with control or PAPalpha- and PAPgamma-specific siRNAs. Histone H3 is shown as a loading control. Image collected and cropped by CiteAb from the following publication (https://mcb.asm.org/content/35/13/2218), licensed under a CC-BY license.

Western Blot: PAPOLG Antibody [NBP1-30060] -

Western Blot: PAPOLG Antibody [NBP1-30060] - An internal A-tract does not promote splicing in the absence of a 3′-terminal poly(A) tail. (A) Schematics of betaWT & betaAnMEN beta delta plasmids, with the positions of the internal A-tracts within exon 3 indicated. Other symbols are the same as in Fig. 1A. (B) RNA immunoprecipitation to assay the level of unspliced beta-globin RNAs bound by PABPN1 in HeLa cells transiently transfected with the betaWT, betaMEN beta delta, betaA20MEN beta delta, betaA40MEN beta delta, or betaA60MEN beta delta construct. Values are expressed as % input following normalization to PSMB3 pre-mRNA levels. (C) RT-qPCR analysis of beta-globin exon 2 & 3 splicing in HeLa cells transiently transfected with the betaWT, betaMEN beta delta, betaA20MEN beta delta, betaA40MEN beta delta, or betaA60MEN beta delta construct. The level of each RNA species was quantitated relative to that recovered from cells transfected with the betaWT construct, which was given a value of 1. (D) Western blot analysis of PAP alpha & PAP gamma proteins in cells treated with control or PAP alpha- & PAP gamma-specific siRNAs. Histone H3 is shown as a loading control. (E) RT-qPCR analysis of exon 2 & 3 splicing in betaWT & betaA46 delta cells treated with control or PAP alpha- & PAP gamma-specific siRNAs. The level of each RNA species is shown as the fold change compared to the level in control siRNA-treated cells after normalization to GAPDH mRNA. All error bars represent standard deviations for at least three biological replicates. *, P < 0.05; **, P < 0.01. US, unspliced; Spl, spliced. Image collected & cropped by CiteAb from the following publication (https://www.tandfonline.com/doi/full/10.1128/MCB.00123-15), licensed under a CC-BY license. Not internally tested by Novus Biologicals.