Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 10E1cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pappalysin-1/PAPP-A Antibodies »

Product Summary for Pappalysin-1/PAPP-A Antibody (10E1cc)

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex.

Specificity

Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and proMBP subunits).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Pappalysin-1/PAPP-A Antibody (10E1cc)

Western Blot: Pappalysin-1/PAPP-A Antibody (10E1cc) [NB120-8346]

Western Blot: Pappalysin-1/PAPP-A Antibody (10E1cc) [NB120-8346]

Western Blot: PAPP A Antibody (10E1) [NB120-8346] - Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blotting. Lane 1: 18A10 (NB100-73059) Lane 2: 7A6 (NB100-73060) Lane 3: 3C8 (NB200-402) Lane 4: 5H9 (proMBP-specific) (NB110-8423) Lane 5: 11E4 (proMBP-specific) (NB120-14597) Lane 6: 7A6 (NB100-73060) Lane 7: 10A5 (NB200-402) Lane 8: 3C8 (NB200-403) Lane 9: 10E1 (NB120-8346) Lanes 1-5: after SDS-PAGE in reduction conditions. Lanes 6-9: Non-reducing conditions. Heterotetrameric complex was detected by anti-PAPP-A MAbs

Applications for Pappalysin-1/PAPP-A Antibody (10E1cc)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays and immunoprecipitation. Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A) (capture -detection): NB100-73058 - NB120-8346, NB120-8346 - NB100-73060.

Recognizes htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB120-8346 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pappalysin-1/PAPP-A

PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).

Long Name

Pregnancy-associated Plasma Protein 1

Alternate Names

ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1

Entrez Gene IDs

5069 (Human)

Gene Symbol

PAPPA

Additional Pappalysin-1/PAPP-A Products

Product Documents for Pappalysin-1/PAPP-A Antibody (10E1cc)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Pappalysin-1/PAPP-A Antibody (10E1cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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