Pappalysin-1/PAPP-A Antibody (9) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05116CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-05116AF350

Alexa Fluor 532

NBP1-05116AF532

Alexa Fluor 594

NBP1-05116AF594

Alexa Fluor 750

NBP1-05116AF750

DyLight 594

NBP1-05116DL594

Janelia Fluor 525

NBP1-05116JF525

Janelia Fluor 549

NBP1-05116JF549

Janelia Fluor 585

NBP1-05116JF585

Janelia Fluor 635

NBP1-05116JF635

Janelia Fluor 646

NBP1-05116JF646

Janelia Fluor 669

NBP1-05116JF669

mFluor Violet 450 SE

NBP1-05116MFV450

mFluor Violet 500 SE

NBP1-05116MFV500

mFluor Violet 610 SE

NBP1-05116MFV610

PerCP

NBP1-05116PCP

Forumulation

Catalog #

BSA Free

NBP1-05116

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pappalysin-1/PAPP-A Antibodies »

Product Summary for Pappalysin-1/PAPP-A Antibody (9) [CoraFluor™ 1]

Immunogen

Purified human PAPP-A

Epitope

The epitope is located on the PAPP-A subunit of the heterotetramer.

Specificity

Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Pappalysin-1/PAPP-A Antibody (9) [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Pappalysin-1/PAPP-A

PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).

Long Name

Pregnancy-associated Plasma Protein 1

Alternate Names

ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1

Gene Symbol

PAPPA

Additional Pappalysin-1/PAPP-A Products

Product Documents for Pappalysin-1/PAPP-A Antibody (9) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Pappalysin-1/PAPP-A Antibody (9) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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